MADD rabbit pAb from ELK Biotechnology Read more ›
MADD rabbit pAb is a research-use antibody from ELK Biotechnology for studying MADD in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. The apoptotic signal is transduced inside these cells by cytoplasmic adaptor proteins. The protein encoded by this gene is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008],
| Specificity | MADD Polyclonal Antibody detects endogenous levels of MADD protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using MADD Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of Jurkat cells using MADD Polyclonal Antibody diluted at 1:1000 | Immunofluorescence analysis of A549 cells, using MADD Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MADD Antibody. The picture on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES2739 |
| Target | MADD |
| Research areas | Antibody Research |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MADD. AA range:751-800 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in testis, ovary, brain and heart (PubMed:8988362). Expressed in spleen, thymus, prostate, testis, ovary, small instestine and colon (PubMed:9115275). Expressed in liver (PubMed:9796103). ; Isoform 1: Not detected in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid. ; Isoform 2: Expressed in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid. ; Isoform 3: Expressed in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid. ; Isoform 4: Expressed in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid. ; Isoform 5: Expressed in the brain, breast, kidney, lung, ovary, pancreas, testis, uterus, stomach and thyroid. ; Isoform 6: Not detec |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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