Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM97229

Glucagon Receptor rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Glucagon Receptor rabbit pAb from ELK Biotechnology Read more ›

Glucagon Receptor rabbit pAb
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Glucagon Receptor rabbit pAb

Product Description

Glucagon Receptor rabbit pAb is a research-use antibody from ELK Biotechnology for studying Glucagon Receptor in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

glucagon receptor(GCGR) Homo sapiens The protein encoded by this gene is a glucagon receptor that is important in controlling blood glucose levels. Defects in this gene are a cause of non-insulin-dependent diabetes mellitus (NIDDM).[provided by RefSeq, Jan 2010],

Additional antibody information

Specificity Glucagon Receptor Polyclonal Antibody detects endogenous levels of Glucagon Receptor protein.
Validation evidence captions Western Blot analysis of various cells using Glucagon Receptor Polyclonal Antibody | Western Blot analysis of COLO205 cells using Glucagon Receptor Polyclonal Antibody | Immunofluorescence analysis of HeLa cells, using GLR Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from COLO205 cells, using GLR Antibody. The lane on the right is blocked with the synthesized peptide.

Key Facts

Product typePrimary Antibody Target: Glucagon Receptor
Collaborator ELK Biotechnology
Partner model ES2433
Target Glucagon Receptor
Research areas>>Neuroactive ligand-receptor interaction, >>Glucagon signaling pathway
Reactivity Human,Mouse,Rat
Applications WB,IF,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human GLR. AA range:95-144
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Liver,Placenta,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
Glucagon Receptor
Also known as
GCGR; Glucagon receptor; GL-R
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIFELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human GLR. AA range:95-144
Observed band
54kD
Cellular localization
Cell membrane ; Multi-pass membrane protein . Is rapidly internalized after ligand-binding. .
Dilution range
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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