FH rabbit pAb from ELK Biotechnology Read more ›
FH rabbit pAb is a research-use antibody from ELK Biotechnology for studying FH in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008],
| Specificity | The antibody detects endogenous fumarase proteins. |
|---|---|
| Validation evidence captions | Western blot analysis of 1) Hela, 2) Mouse Brain, 3) HepG2, diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
| Collaborator | ELK Biotechnology |
| Partner model | ES20754 |
| Target | FH |
| Research areas | >>Citrate cycle (TCA cycle), >>Pyruvate metabolism, >>Metabolic pathways, >>Carbon metabolism, >>Cushing syndrome, >>Pathways in cancer, >>Renal cell carcinoma |
| Reactivity | Human,Mouse,Rat |
| Applications | WB |
| Conjugation | Unconjugated |
| Dilution range | WB: 1:1000 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant Protein of Fumarate hydratase, mitochondrial |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in red blood cells; underexpressed in red blood cells (cytoplasm) of patients with hereditary non-spherocytic hemolytic anemia of unknown etiology. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB: 1:1000 | — |
| Mouse | — | — |
| Rat | — | — |
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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