Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM94329

TAZ (phospho-Ser89) rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

TAZ (phospho-Ser89) rabbit pAb from ELK Biotechnology Read more ›

TAZ (phospho-Ser89) rabbit pAb
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TAZ (phospho-Ser89) rabbit pAb

Product Description

TAZ (phospho-Ser89) rabbit pAb is a research-use antibody from ELK Biotechnology for studying TAZ in WB workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Additional antibody information

Specificity This antibody detects endogenous levels of Human Mouse Rat TAZ (phospho-Ser89)
Validation evidence captions Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: TAZ
Collaborator ELK Biotechnology
Partner model ES12809
Target TAZ
Research areas>>Hippo signaling pathway, >>Hippo signaling pathway - multiple species
Reactivity Human,Mouse,Rat
Applications WB
ConjugationUnconjugated
Dilution rangeWB 1:1000-2000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized phosho peptide around human TAZ (Ser89)
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Assay type Antibody validation/application
Expression system Highly expressed in kidney, heart, placenta and lung. Expressed in the thyroid tissue.
Purity The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1000-2000
Mouse
Rat

Specifications

Target
TAZ
Also known as
WW domain-containing transcription regulator protein 1 (Transcriptional coactivator with PDZ-binding motif)
Concentration
1 mg/ml
Working concentration
WB 1:1000-2000
Purity
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WB
Immunogen
Synthesized phosho peptide around human TAZ (Ser89)
Observed band
44kD
Cellular localization
Nucleus . Cytoplasm . Cell membrane . Concentrates along specific portions of the plasma membrane, and accumulates in punctate nuclear bodies (By similarity). When phosphorylated, is retained in the cytoplasm by YWHAZ (By similarity). Can be retained in the nucleus by MED15 (PubMed:18568018). Localized in the cytoplasm in areas of epithelial cell high density (PubMed:21145499). At blastocyst stage expressed in the nucleus in trophectodermal cells, however expressed in the cytoplasm in the inner cell mass (By similarity). .
Dilution range
WB 1:1000-2000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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