Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92394

α-tubulin Mouse mAb

ISO 9001|ABMIUM Verified™|Antibody

α-tubulin Mouse mAb from ELK Biotechnology Read more ›

α-tubulin Mouse mAb
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α-tubulin Mouse mAb

Product Description

α-tubulin Mouse mAb is a research-use antibody from ELK Biotechnology for studying α-tubulin in WB, IHC, IF, IP workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 kiloDaltons. Microtubules are assembled from dimers of α- and β-tubulin.

Additional antibody information

Validation evidence captions Western blot analysis of 1) Hela, 2) Rat BrianTissue, 3) Mouse Brain Tissue with EM1034 diluted at 1:5,000. | Immunohistochemical analysis of paraffin-embedded human Colon caricnoma using α tubulin (EM1034)Mouse mAb diluted at 1:500. | 1、Input: Mouse Brain Tissue Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1034 1:5,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific (S003), 1:5,000 | IF analysis of Hela with EM1034(Left) and DAPI (Right) diluted at 1:100.

Key Facts

Product typePrimary Antibody Target: α-tubulin
Collaborator ELK Biotechnology
Partner model EM1034
Target α-tubulin
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC,IF,IP
ConjugationUnconjugated
Dilution rangeWB 1:5,000-10,000 IP 1:200 IF 1:100-200 IHC 1:200-500
Host Mouse
Clonality Monoclonal
Isotype IgG1
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:5
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes
ABMIUM Validated™ IF
Species Dilution Notes
ABMIUM Validated™ IP
Species Dilution Notes

Specifications

Target
α-tubulin
Also known as
alpha tubulin 2, B alpha 1, H2 alpha, Hum a tub 1, TUBA1, TUBA1A,Tubulin alpha 1chain
Concentration
1mg/mL
Working concentration
WB 1:5,000-10,000 IP 1:200 IF 1:100-200 IHC 1:200-500
Form / buffer
Liquid
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHCIFIP
Immunogen
Recombinant Protein
Observed band
52kDa
Cellular localization
Cytoplasm,Cytoskeleton,Microtubule
Dilution range
WB 1:5,000-10,000 IP 1:200 IF 1:100-200 IHC 1:200-500

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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