Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92335

HIF-1α Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

HIF-1α Rabbit pAb from ELK Biotechnology Read more ›

HIF-1α Rabbit pAb
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HIF-1α Rabbit pAb

Product Description

HIF-1α Rabbit pAb is a research-use antibody from ELK Biotechnology for studying HIF-1α in WB, IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Hypoxia-inducible factor 1-alpha, also known as HIF-1-alpha, is a protein that in humans is encoded by the HIF1A gene. HIF-1-alpha found in mammalian cells growing at low oxygen concentrations. It plays an essential role in cellular and systemic responses to hypoxia.

Additional antibody information

Validation evidence captions Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HIF-1α(EA335)Rabbit pAb diluted at 1:200 | Western blot analysis of 1) Mouse Liver Tissue Lysate, 2)Rat Liver Tissue Lysate, 3) HepG2 Cell Lysate using HIF-1α (EA335)Rabbit pAb diluted at 1:1000.

Key Facts

Product typePrimary Antibody Target: HIF-1α
Collaborator ELK Biotechnology
Partner model EA335
Target HIF-1α
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:500-1,000 IHC 1:100-200
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-1
Mouse
Rat
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
HIF-1α
Also known as
Hypoxia inducibel factor 1 alpha, HIF1A, ARNT, MOP1, PAS domain-containing protein 8, PASD8
Concentration
1mg/mL
Working concentration
WB 1:500-1,000 IHC 1:100-200
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
120kDa
Cellular localization
Cytoplasm, Nucleus
Dilution range
WB 1:500-1,000 IHC 1:100-200

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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