Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92196

LC3B Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

LC3B Rabbit pAb from ELK Biotechnology Read more ›

LC3B Rabbit pAb
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LC3B Rabbit pAb

Product Description

LC3B Rabbit pAb is a research-use antibody from ELK Biotechnology for studying LC3B in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Autophagy marker Light Chain 3 (LC3) was originally identified as a subunit of microtubule-associated proteins 1A and 1B (termed MAP1LC3).

Additional antibody information

Validation evidence captions Western blot analysis of 1) Hela, 2) 3T3, 3)Rat Brain Tissue with LC3B Rabbit pAb EA174 diluted at 1:2,000. | Immunohistochemical analysis of paraffin-embedded Rat BrainTissue using LC3B(EA174)Rabbit pAb diluted at 1:500.

Key Facts

Product typePrimary Antibody Target: LC3B
Collaborator ELK Biotechnology
Partner model EA174
Target LC3B
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000-2,000 IHC 1:200-500
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
LC3B
Also known as
ATG8F antibody, MAP1 light chain 3 like protein 2 antibody, MAP1A/MAP1B LC3 B antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:200-500
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
14,16kDa
Cellular localization
Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Microtubule
Dilution range
WB 1:1,000-2,000 IHC 1:200-500

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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