Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92171

Histone H2A.X(Acetyl Lys5) Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

Histone H2A.X(Acetyl Lys5) Rabbit pAb from ELK Biotechnology Read more ›

Histone H2A.X(Acetyl Lys5) Rabbit pAb
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Histone H2A.X(Acetyl Lys5) Rabbit pAb

Product Description

Histone H2A.X(Acetyl Lys5) Rabbit pAb is a research-use antibody from ELK Biotechnology for studying Histone H2A.X(Acetyl Lys5) in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.

Additional antibody information

Validation evidence captions Western blot analysis of extracts from Hela cells, untreated (-) or treated with TSA (1µM, 18 hr; +), using Acetyl-Histone H2A.X (Lys5) Rabbit pAb (upper) EA116 1:2,000 and β-actin Mouse mAb (lower) EM1031 1:5,000. | Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using Histone H2A.X (Acetyl Lys5)(E116)Rabbit pAb diluted at 1:500.

Key Facts

Product typePrimary Antibody Target: Histone H2A.X(Acetyl Lys5)
Collaborator ELK Biotechnology
Partner model EA116
Target Histone H2A.X(Acetyl Lys5)
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000-2,000 IHC 1:200-500
Host Rabbit
Clonality Polyclonal
Isotype Antiserum
Immunogen Synthetic Peptide
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
Histone H2A.X(Acetyl Lys5)
Also known as
H2A histone family member X antidoby, H2AFX antibody, H2AX Histoneh antibody, Histone H2AX antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-2,000 IHC 1:200-500
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
Antiserum
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHC
Immunogen
Synthetic Peptide
Observed band
15kDa
Cellular localization
Nucleus,Chromosome
Dilution range
WB 1:1,000-2,000 IHC 1:200-500

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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