Histone H2A.X(Acetyl Lys5) Rabbit pAb from ELK Biotechnology Read more ›
Histone H2A.X(Acetyl Lys5) Rabbit pAb is a research-use antibody from ELK Biotechnology for studying Histone H2A.X(Acetyl Lys5) in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
Histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.
| Validation evidence captions | Western blot analysis of extracts from Hela cells, untreated (-) or treated with TSA (1µM, 18 hr; +), using Acetyl-Histone H2A.X (Lys5) Rabbit pAb (upper) EA116 1:2,000 and β-actin Mouse mAb (lower) EM1031 1:5,000. | Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using Histone H2A.X (Acetyl Lys5)(E116)Rabbit pAb diluted at 1:500. |
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| Collaborator | ELK Biotechnology |
| Partner model | EA116 |
| Target | Histone H2A.X(Acetyl Lys5) |
| Research areas | Antibody Research |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC |
| Conjugation | Unconjugated |
| Dilution range | WB 1:1,000-2,000 IHC 1:200-500 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Antiserum |
| Immunogen | Synthetic Peptide |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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