Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM100329

PDE2A rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

PDE2A rabbit pAb from ELK Biotechnology Read more ›

PDE2A rabbit pAb
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PDE2A rabbit pAb

Product Description

PDE2A rabbit pAb is a research-use antibody from ELK Biotechnology for studying PDE2A in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

alternative products:Additional isoforms seem to exist. Experimental confirmation may be lacking for some isoforms,catalytic activity:Nucleoside 3',5'-cyclic phosphate + H(2)O = nucleoside 5'-phosphate.,function:Hydrolyzes both cyclic AMP (cAMP) and cyclic GMP (cGMP).,similarity:Belongs to the cyclic nucleotide phosphodiesterase family.,similarity:Contains 2 GAF domains.,subunit:Homodimer.,tissue specificity:Expressed in brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney and pancreas.,

Additional antibody information

Specificity PDE2A Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western Blot analysis of Hela lysis, using primary antibody at 1:1000 dilution. Secondary antibody(catalog#:RS23920) was diluted at 1:10000

Key Facts

Product typePrimary Antibody Target: PDE2A
Collaborator ELK Biotechnology
Partner model ES9528
Target PDE2A
Research areas>>Purine metabolism, >>Metabolic pathways, >>cGMP-PKG signaling pathway, >>Olfactory transduction, >>Aldosterone synthesis and secretion, >>Morphine addiction
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWB 1:500-2000 ELISA 1:5000-20000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from part region of human protein
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Widely expressed (PubMed:9210593, PubMed:29392776). Expressed in brain, with high expression observed in the corpus striatum (PubMed:29392776). Also expressed in heart, placenta, lung, skeletal muscle, kidney and pancreas (PubMed:9210593, PubMed:29392776).
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:500-2000 ELISA 1:5000-20000
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
PDE2A
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from part region of human protein
Observed band
103kD
Cellular localization
Isoform PDE2A3: Cell membrane ; Lipid-anchor .; Isoform PDE2A2: Mitochondrion matrix . Mitochondrion inner membrane . Mitochondrion outer membrane .; Isoform PDE2A1: Cytoplasm .; Isoform 5: Mitochondrion .
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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