Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM99732

ACYP2 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

ACYP2 rabbit pAb from ELK Biotechnology Read more ›

ACYP2 rabbit pAb 50µL/100µL
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ACYP2 rabbit pAb 50µL/100µL

Product Description

ACYP2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ACYP2 in WB, ELISA workflows with listed reactivity for Human, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Acylphosphatase can hydrolyze the phosphoenzyme intermediate of different membrane pumps, particularly the Ca2+/Mg2+-ATPase from sarcoplasmic reticulum of skeletal muscle. Two isoenzymes have been isolated, called muscle acylphosphatase and erythrocyte acylphosphatase on the basis of their tissue localization. This gene encodes the muscle-type isoform (MT). An increase of the MT isoform is associated with muscle differentiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2016],

Additional antibody information

Specificity ACYP2 Polyclonal Antibody detects endogenous levels of ACYP2 protein.
Validation evidence captions Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°over night

Key Facts

Product typePrimary Antibody Target: ACYP2
Collaborator ELK Biotechnology
Partner model ES8100
Target ACYP2
Research areas>>Pyruvate metabolism, >>Metabolic pathways
Reactivity Human,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Synthesized peptide derived from ACYP2 . at AA range: 40-120
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Heart,Muscle,Ovary,
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

2 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
ACYP2
Also known as
ACYP2; ACYP; Acylphosphatase-2; Acylphosphatase; muscle type isozyme; Acylphosphate phosphohydrolase 2
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat
Tested applications
WBELISA
Immunogen
Synthesized peptide derived from ACYP2 . at AA range: 40-120
Observed band
18kD
Cellular localization
mitochondrion,
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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