IL-12B rabbit pAb from ELK Biotechnology Read more ›
IL-12B rabbit pAb is a research-use antibody from ELK Biotechnology for studying IL-12B in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. [provi
| Specificity | IL-12B Polyclonal Antibody detects endogenous levels of IL-12B protein. |
|---|---|
| Validation evidence captions | Western blot analysis of lysate from K562 cells, using IL-12B antibody. |
| Collaborator | ELK Biotechnology |
| Partner model | ES5895 |
| Target | IL-12B |
| Research areas | >>Cytokine-cytokine receptor interaction, >>Toll-like receptor signaling pathway, >>RIG-I-like receptor signaling pathway, >>C-type lectin receptor signaling pathway, >>JAK-STAT signaling pathway, >>Th1 and Th2 cell differentiation, >>Alcoholic liver disease, >>Type I diabetes mellitus, >>Pertussis, >>Legionellosis, >>Leishmaniasis, >>Chagas disease, >>African trypanosomiasis, >>Toxoplasmosis, >>Amoebiasis, >>Tuberculosis, >>Measles, >>Influenza A, >>Herpes simplex virus 1 infection, >>Coronavirus disease - COVID-19, >>Pathways in cancer, >>Proteoglycans in cancer, >>Inflammatory bowel disease, >>Allograft rejection, >>Lipid and atherosclerosis |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IL-12B. AA range:228-277 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Brain, |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
No customer reviews yet.
