AR-α1A rabbit pAb from ELK Biotechnology Read more ›
AR-α1A rabbit pAb is a research-use antibody from ELK Biotechnology for studying AR-α1A in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This gene encodes alpha-1A-adrenergic receptor. Alternative splicing of this gene generates four transcript variants, which encode four different isoforms with distinct C-termini but having similar ligand binding properties. [provided by RefSeq, Jul 2008],
| Specificity | AR-α1A Polyclonal Antibody detects endogenous levels of AR-α1A protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of JK cells using AR-α1A Polyclonal Antibody | Immunofluorescence analysis of A549 cells, using ADRA1A Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of ADRA1A Antibody. The lane on the right is blocked with the ADRA1A peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES4879 |
| Target | AR-α1A |
| Research areas | >>Calcium signaling pathway, >>cGMP-PKG signaling pathway, >>Neuroactive ligand-receptor interaction, >>AMPK signaling pathway, >>Adrenergic signaling in cardiomyocytes, >>Vascular smooth muscle contraction, >>Salivary secretion |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ADRA1A. AA range:136-185 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Expressed in heart, brain, liver and prostate, but not in kidney, lung, adrenal, aorta and pituitary. Within the prostate, expressed in the apex, base, periurethral and lateral lobe. Isoform 4 is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart. |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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