TGFβ RI rabbit pAb from ELK Biotechnology Read more ›
TGFβ RI rabbit pAb is a research-use antibody from ELK Biotechnology for studying TGF β Receptor I in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF-beta signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys-Dietz aortic aneurysm syndrome (LDAS). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008],
| Specificity | TGFβ RI Polyclonal Antibody detects endogenous levels of TGFβ RI protein. |
|---|---|
| Validation evidence captions | Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min. | Western Blot analysis of various cells using TGFβ RI Polyclonal Antibody diluted at 1:500 | Western Blot analysis of COLO205 cells using TGFβ RI Polyclonal Antibody diluted at 1:500 | Immunofluorescence analysis of HeLa cells, using TGF beta Receptor I Antibody. The picture on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES3594 |
| Target | TGF β Receptor I |
| Research areas | >>MAPK signaling pathway, >>Cytokine-cytokine receptor interaction, >>FoxO signaling pathway, >>Endocytosis, >>Cellular senescence, >>TGF-beta signaling pathway, >>Apelin signaling pathway, >>Osteoclast differentiation, >>Hippo signaling pathway, >>Adherens junction, >>Th17 cell differentiation, >>Relaxin signaling pathway, >>AGE-RAGE signaling pathway in diabetic complications, >>Chagas disease, >>Hepatitis B, >>Human T-cell leukemia virus 1 infection, >>Pathways in cancer, >>Colorectal cancer, >>Pancreatic cancer, >>Chronic myeloid leukemia, >>Hepatocellular carcinoma, >>Gastric cancer, >>Diabetic cardiomyopathy |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IF,ELISA |
| Conjugation | Unconjugated |
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TGF beta Receptor I. AA range:131-180 |
| Concentration | 1 mg/ml |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Assay type | Antibody validation/application |
| Expression system | Found in all tissues examined, most abundant in placenta and least abundant in brain and heart. Expressed in a variety of cancer cell lines (PubMed:25893292). |
| Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
| Species | Dilution | Notes |
|---|---|---|
| Human | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications. | — |
| Mouse | — | — |
| Rat | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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