Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM96928

CLC-7 rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

CLC-7 rabbit pAb from ELK Biotechnology Read more ›

CLC-7 rabbit pAb
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CLC-7 rabbit pAb

Product Description

CLC-7 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CLC-7 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

chloride voltage-gated channel 7(CLCN7) Homo sapiens The product of this gene belongs to the CLC chloride channel family of proteins. Chloride channels play important roles in the plasma membrane and in intracellular organelles. This gene encodes chloride channel 7. Defects in this gene are the cause of osteopetrosis autosomal recessive type 4 (OPTB4), also called infantile malignant osteopetrosis type 2 as well as the cause of autosomal dominant osteopetrosis type 2 (OPTA2), also called autosomal dominant Albers-Schonberg disease or marble disease autosoml dominant. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. OPTA2 is the most common form of osteopetrosis, occurring in adolescence or adulthood. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity CLC-7 Polyclonal Antibody detects endogenous levels of CLC-7 protein.
Validation evidence captions Western Blot analysis of A549 cells using CLC-7 Polyclonal Antibody diluted at 1:500 | Western blot analysis of CLCN7 Antibody. The lane on the right is blocked with the CLCN7 peptide. | Western blot analysis of the lysates from COLO205 cells using CLCN7 antibody.

Key Facts

Product typePrimary Antibody Target: CLC-7
Collaborator ELK Biotechnology
Partner model ES1993
Target CLC-7
Research areasAntibody Research
Reactivity Human,Mouse,Rat
Applications WB,ELISA
ConjugationUnconjugated
Dilution rangeWestern Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen The antiserum was produced against synthesized peptide derived from human CLCN7. AA range:10-59
Concentration 1 mg/ml
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Assay type Antibody validation/application
Expression system Brain and kidney.
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
ABMIUM Validated™ WB
Species Dilution Notes
Human Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Mouse
Rat
ABMIUM Validated™ ELISA
Species Dilution Notes

Specifications

Target
CLC-7
Also known as
CLCN7; H(+)/Cl(-) exchange transporter 7; Chloride channel 7 alpha subunit; Chloride channel protein 7; ClC-7
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Mouse,Rat
Tested applications
WBELISA
Immunogen
The antiserum was produced against synthesized peptide derived from human CLCN7. AA range:10-59
Observed band
90kD
Cellular localization
Lysosome membrane ; Multi-pass membrane protein .
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Stability

Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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