NFkB p65 Mouse mAb from ELK Biotechnology Read more ›
NFkB p65 Mouse mAb is a research-use antibody from ELK Biotechnology for studying NFkB p65 in WB, IHC, IF, IP workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of immune function required for resistance to infection.
| Validation evidence captions | Western blot analysis of extracts from HeLa (Lane 1),MCF-7 (Lane 2), Mouse Liver (Lane 3), Rat Kidney (Lane 4), using NFκB p65 diluted at 1:1,500. | 1、Input: Hela Cell Lysate 2、IP product: IP dilute 1:200 Western blot analysis: primary antibody : EM1111 1:2,000 Secondary antibody: Goat anti-Mouse IgG, Light chain specific(S003), 1:5,000 | IF analysis of Hela with EM1111(Left) and DAPI (Right) diluted at 1:100. | Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using NFkB p65 （EM1111）Mouse mAbdiluted at 1:500. |
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| Collaborator | ELK Biotechnology |
| Partner model | EM1111 |
| Target | NFkB p65 |
| Research areas | Antibody Research |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC,IF,IP |
| Conjugation | Unconjugated |
| Dilution range | WB 1:500-2,000 IP 1:200 IF 1:100-200 IHC 1:200-500 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Recombinant Protein |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Rat | |||||||
| Mouse |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:500-2 | — |
| Rat | — | — |
| Mouse | — | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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