EGFR Mouse mAb from ELK Biotechnology Read more ›
EGFR Mouse mAb is a research-use antibody from ELK Biotechnology for studying EGFR in WB, IHC, IF workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascadesβ-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against β-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Actin may not be stable in certain cells. For example, expression of β-Actin in adipose tissue is very low and therefore β-Actin should not be used as loading control for these tissues.
| Validation evidence captions | Western blot analysis of Hela with EGFR mAb diluted at 1:1,000. | IF analysis of Hela with EM1044(Left) and DAPI (Right) diluted at 1:100. | Immunohistochemical analysis of paraffin-embedded Human Lung caricnoma using EGFR （EM1044）Mouse mAb diluted at 1:500. |
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| Collaborator | ELK Biotechnology |
| Partner model | EM1044 |
| Target | EGFR |
| Research areas | Antibody Research |
| Reactivity | Human |
| Applications | WB,IHC,IF |
| Conjugation | Unconjugated |
| Dilution range | WB 1:1,000 IF 1;100-200 IHC 1:200-500 |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Immunogen | Synthetic Peptide |
| Concentration | 1mg/mL |
| Assay type | Antibody validation/application |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human |
| Species | Dilution | Notes |
|---|---|---|
| Human | WB 1:1 | — |
| Species | Dilution | Notes |
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| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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