Collaborator ELK Biotechnology
Primary AntibodyISO 9001:2015
ABM92157

GAP43 Rabbit pAb

ISO 9001|ABMIUM Verified™|Antibody

GAP43 Rabbit pAb from ELK Biotechnology Read more ›

GAP43 Rabbit pAb
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GAP43 Rabbit pAb

Product Description

GAP43 Rabbit pAb is a research-use antibody from ELK Biotechnology for studying GAP43 in WB, IHC workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Growth Associated Protein 43 also known as GAP43 is a protein that in humans is encoded by the GAP43 gene. GAP43, is a nervous tissue-specific cytoplasmic protein that can be attached to the membrane via a dual palmitoylation sequence on cysteines 3 and 4. This sequence targets GAP43 to lipid rafts.

Additional antibody information

Validation evidence captions Western blot analysis of 1) Hela, 2)Mouse Brain, 3) Rat Brain tissue with EA051 diluted at 1:3,000. | Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using α-Smooth Muscle Actin (EA051) Rabbit pAb diluted at 1:500.

Key Facts

Product typePrimary Antibody Target: GAP43
Collaborator ELK Biotechnology
Partner model EA051
Target GAP43
Research areasAntibody Research
Reactivity Human,Rat,Mouse
Applications WB,IHC
ConjugationUnconjugated
Dilution rangeWB 1:1,000-3,000 IHC 1:500-1,000
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Recombinant Protein
Concentration 1mg/mL
Assay type Antibody validation/application
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Application Validation Matrix

3 speciess
ABMIUM Validated™ ABMIUM Verified™ Expected Performance Not suitable
SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
ABMIUM Validated™ WB
Species Dilution Notes
Human WB 1:1
Rat
Mouse
ABMIUM Validated™ IHC
Species Dilution Notes

Specifications

Target
GAP43
Also known as
B50 antibody, Growth Associated Protein 43 antibody, Nerve Growth Related Peptide antibody
Concentration
1mg/mL
Working concentration
WB 1:1,000-3,000 IHC 1:500-1,000
Form / buffer
Liquid
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Species reactivity
Human,Rat,Mouse
Tested applications
WBIHC
Immunogen
Recombinant Protein
Observed band
38,43kDa
Cellular localization
Cell junction, Cell membrane, Cell projection, Membrane, Synapse
Dilution range
WB 1:1,000-3,000 IHC 1:500-1,000

Storage & Stability

Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Compliance & Certifications

ABMIUM
ABMIUM Verified

Collaborator meets ABMIUM quality standards and overall product performance standards have been met.

ISO
ISO 9001

Quality management certification.

RUO
Research Use Only

For research applications. Not for diagnostic use.

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