{"product_id":"elk-es2515-hisrs-rabbit-pab","title":"HisRS rabbit pAb","description":"\u003cp\u003eHisRS rabbit pAb is a research-use antibody from ELK Biotechnology for studying HisRS in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.\u003c\/p\u003e\n\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eAminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis\/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012],\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003eHisRS Polyclonal Antibody detects endogenous levels of HisRS protein.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eWestern Blot analysis of various cells using HisRS Polyclonal Antibody diluted at 1:2000 | Immunofluorescence analysis of HepG2 and HeLa cells, using HARS Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from COLO cells, using HARS Antibody. The lane on the right is blocked with the synthesized peptide. | Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56186182992212,"sku":"ABM107134","price":220.0,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56186183024980,"sku":"ABM97270","price":132.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES2515-c-1.jpg?v=1782043530","url":"https:\/\/www.abmium.com\/zh\/products\/elk-es2515-hisrs-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}