{"product_id":"elk-es1304-eif2alpha-phospho-ser51-rabbit-pab","title":"eIF2α (phospho Ser51) rabbit pAb","description":"\u003cp\u003eeIF2α (phospho Ser51) rabbit pAb is a research-use antibody from ELK Biotechnology for studying eIF2α in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat, Dog, Pig, Fish. Specifications and supporting documents below should be reviewed when planning the experiment.\u003c\/p\u003e\n\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eThe translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010],\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003ePhospho-eIF2α (S51) Polyclonal Antibody detects endogenous levels of eIF2α protein only when phosphorylated at S51.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eImmunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H\u0026amp;L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56185278562644,"sku":"ABM104340","price":220.0,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56185278595412,"sku":"ABM94476","price":132.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES1304-c-1.jpg?v=1782033642","url":"https:\/\/www.abmium.com\/tr\/products\/elk-es1304-eif2alpha-phospho-ser51-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}