{"product_id":"rabbit-anti-goat-igg-h-l-secondary-antibody-ba1006","title":"Rabbit Anti-Goat IgG (H+L) Secondary Antibody","description":"\u003ch2\u003eProduct Overview\u003c\/h2\u003e \u003ctable class=\"data-table\" attributes_table\u003e \u003ctr\u003e \u003cth\u003eProduct Name\u003c\/th\u003e \u003ctd\u003eRabbit Anti-Goat IgG (H+L) Secondary Antibody, Biotin Conjugate\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eSynonyms\u003c\/th\u003e \u003ctd\u003eBiotin-conjugated Rabbit Anti-Goat IgG; Rabbit Anti-Goat IgG Biotinylated Antibody; Biotinylated Rabbit Anti-Goat IgG Secondary Antibody; Rabbit Anti-Goat IgG Secondary Antibody, Biotin-labeled\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eDescription\u003c\/th\u003e \u003ctd\u003eRabbit Anti-Goat IgG (H+L) Secondary Antibody, Biotin Conjugate, for in sensitive immunodetection and\/or quantification of low-abundance target proteins through ELISA or IHC by using reporter-labeled biotin-binding signal amplification systems\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eReagent Type\u003c\/th\u003e \u003ctd\u003eBiotin conjugated secondary antibody\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eConjugate\u003c\/th\u003e \u003ctd\u003eBiotin\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eHost\u003c\/th\u003e \u003ctd\u003eRabbit\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eTarget Species\u003c\/th\u003e \u003ctd\u003eGoat\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eAntibody Class\u003c\/th\u003e \u003ctd\u003eIgG\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eClonality\u003c\/th\u003e \u003ctd\u003ePolyclonal\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eImmunogen\u003c\/th\u003e \u003ctd\u003eWhole molecule goat IgG\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003ePurification\u003c\/th\u003e \u003ctd\u003eImmunoaffinity chromatography\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eSpecificity\u003c\/th\u003e \u003ctd\u003eGoat IgG specific; no cross-reactivity with human\/rat\/mouse\/rabbit IgG\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eForm Supplied\u003c\/th\u003e \u003ctd\u003eLiquid: concentrated buffered stock solution\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eFormulation\u003c\/th\u003e \u003ctd\u003e0.5 mg biotin-conjugated secondary antibody \u003cbr\u003e0.01 M PBS (PH 7.4) \u003cbr\u003e50% glycerol\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003ePack Size\u003c\/th\u003e \u003ctd\u003e0.5 ml\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eConcentration\u003c\/th\u003e \u003ctd\u003e1 mg\/ml\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eApplication\u003c\/th\u003e \u003ctd\u003eELISA, IHC\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eStorage\u003c\/th\u003e \u003ctd\u003eAt -20?C for one year from date of receipt. Avoid repeated freezing and thawing.\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003ePrecautions\u003c\/th\u003e \u003ctd\u003eFOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE\u003c\/td\u003e \u003c\/tr\u003e \u003c\/table\u003e \u003ch2\u003eAssay Information\u003c\/h2\u003e \u003ctable class=\"data-table\" attributes_table\u003e \u003ctr\u003e \u003cth\u003eSample Type\u003c\/th\u003e \u003ctd\u003eHuman primary-antibody-probed: SDS-PAGE separated-, membrane-immobilized-proteins from cell\/tissue lysates, formalin-fixed paraffin-embedded (FFPE) tissue sections on slides\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eAssay Type\u003c\/th\u003e \u003ctd\u003eImmunoanalytical\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eTechnique\u003c\/th\u003e \u003ctd\u003eIn immunodetection of target protein via reporter-labeled biotin-binding detection systems\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eAssay Purpose\u003c\/th\u003e \u003ctd\u003eProtein detection\/quantification\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eEquipment Needed\u003c\/th\u003e \u003ctd\u003eWB\/Dot blot\/ELISA\/IHC instrumentation; Reporter signal detectors: X-ray film cassette; a charge-coupled device (CCD) imager; Spectrophotometer; fluorescent or electron microscope\u003c\/td\u003e \u003c\/tr\u003e \u003c\/table\u003e \u003ch2\u003eMain Advantages\u003c\/h2\u003e \u003ctable class=\"data-table\" attributes_table\u003e \u003ctr\u003e \u003cth\u003eSpecific\u003c\/th\u003e \u003ctd\u003eHigh signal-to-noise ratio\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eHigh Signal Amplification\u003c\/th\u003e \u003ctd\u003eMultiple secondary antibodies can bind to a single primary antibody; Multiple reporter molecules localize to a single biotin via avidin\/streptavidin bridges\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eFast\u003c\/th\u003e \u003ctd\u003eFewer processing steps - no need to add a substrate; Less optimization required compared to enzymatic detection; Generates strong signals in a relatively short time span; Fluorescence can be observed ly\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eQuantifieable\u003c\/th\u003e \u003ctd\u003eAllows quantification of detected signal\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eEasy to Use\u003c\/th\u003e \u003ctd\u003eSupplied in a workable liquid format \u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eFlexible\u003c\/th\u003e \u003ctd\u003eBiotin- (Strept)Avidin system can be coupled with various types of reporters (enzymes, fluorochromes, fluorophores, chromophores, etc.); One type of labeled secondary antibody can be used to recognize different types of primary antibodies of the target organism specific to a particular antigen\u003c\/td\u003e \u003c\/tr\u003e \u003ctr\u003e \u003cth\u003eCompatible\u003c\/th\u003e \u003ctd\u003eBiotin does not interfere with catalysis or antibody binding\u003c\/td\u003e \u003c\/tr\u003e \u003c\/table\u003e \u003ch2\u003eBackground\u003c\/h2\u003e \u003cp\u003eMost commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest. Purified secondary antibodies are further solid phase adsorbed with other species serum proteins to minimize cross-reactivity in tissue or cell preparations, and are then modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes\/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application.\u003c\/p\u003e \u003cp\u003eBiotinylated antibodies are widely used in systems where signal amplification is desired. Often 15-20 biotin moieties are coupled to a single IgG secondary antibody. Biotin binds avidin, streptavidin, or neutravidin with a high degree of affinity and specificity. In immunoassays avidin\/streptavidin-biotin binding is used as a bridge between antibodies and reporters like enzymes (HRP, AP), fluorophores, chromophores, etc. Both avidin and streptavidin are tetrameric proteins capable of binding 4 biotin groups to each molecule of avidin or streptavidin, thus amplifying the signal intensity and detection sensitivity by increasing the concentration of reporters at the antigenic site. Two main biotin-binding detection systems have been widely utilized: Avidin-Biotin Complex (ABC) and Labeled Streptavidin Biotin (LSAB) methods. In the ABC method free avidin (or streptavidin) is used as a bridge\/link between the biotinylated antibody and а biotinylated reporter molecule, resulting in three reporter molecules coupled to the biotinylated antibody. The LSAB method employs a reporter-labeled streptavidin (avidin or neutravidin can alternatively be used) to detect the bound biotinylated-secondary antibody on the tissue section, blotting membrane or ELISA plate, improving the sensitivity of detection by 8-fold. The LSAB method is used when the avidin-biotin-enzyme complex in the ABC method becomes too large to penetrate the tissue. \u003c\/p\u003e\u003ch3\u003eProduct information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSupplier\u003c\/th\u003e\n\u003ctd\u003eBoster Bio\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eABMIUM evidence status\u003c\/th\u003e\n\u003ctd\u003eABMIUM Verified™ collaborator-tested data\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalogue reference\u003c\/th\u003e\n\u003ctd\u003eBA1006\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct category\u003c\/th\u003e\n\u003ctd\u003eSecondary Antibodies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReactive species\u003c\/th\u003e\n\u003ctd\u003eGoat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTested applications\u003c\/th\u003e\n\u003ctd\u003eELISA, IHC, IHC-P, WB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAvailable conjugations\u003c\/th\u003e\n\u003ctd\u003eBiotin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003eThis biotin conjugated antibody is specific for goat IgG and shows no cross-reactivity with human\/rat\/mouse\/rabbit IgG.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eImmunogen\u003c\/th\u003e\n\u003ctd\u003eGoat IgG (whole molecule).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHost\u003c\/th\u003e\n\u003ctd\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003ePolyclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eAt -20℃ for one year from date of receipt. Avoid repeated freezing and thawing.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNCBI Gene ID\u003c\/th\u003e\n\u003ctd\u003e6494\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\u003ch3\u003eAvailable variants\u003c\/h3\u003e\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eConjugation\u003c\/th\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin\u003c\/td\u003e\n\u003ctd\u003e0.5ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiotin\u003c\/td\u003e\n\u003ctd\u003e1ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Boster Bio","offers":[{"title":"Biotin \/ 0.5ml","offer_id":56044946522452,"sku":"ABM33925","price":40.0,"currency_code":"GBP","in_stock":true},{"title":"Biotin \/ 1ml","offer_id":56044946555220,"sku":"ABM33925","price":71.0,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/Screenshot_2026-06-08_210431_a71d127a-8dd0-46b1-918b-baf498fe5599.png?v=1780951801","url":"https:\/\/www.abmium.com\/products\/rabbit-anti-goat-igg-h-l-secondary-antibody-ba1006","provider":"ABMIUM","version":"1.0","type":"link"}