{"product_id":"elk-es8619-prc1-phospho-thr481-rabbit-pab","title":"PRC1(Phospho-Thr481)rabbit pAb","description":"\u003cp\u003ePRC1(Phospho-Thr481)rabbit pAb is an ELK Biotechnology antibody for research workflows involving PRC1(Phospho-Thr481)Polyclonal Antibody.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eThis gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2\/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012],\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003eThe antibody detects endogenous PRC1Phospho occurs at Thr481\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eWestern blot analysis of 293T VEC lysate, antibody was diluted at 500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PRC1 (Phospho-Thr481) Antibody | Western blot analysis of lysates from HeLa cells, using p-PRC1 (Phospho-Thr481) antibody. | Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56187438661972,"sku":"ABM109747","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56187438694740,"sku":"ABM99883","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES8619-c-1.jpg?v=1782055132","url":"https:\/\/www.abmium.com\/products\/elk-es8619-prc1-phospho-thr481-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}