{"product_id":"elk-es8464-tle1-2-3-4-rabbit-pab","title":"TLE1\/2\/3\/4 rabbit pAb","description":"\u003cp\u003eTLE1\/2\/3\/4 rabbit pAb is an ELK Biotechnology antibody for research workflows involving TLE1\/2\/3\/4.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003efunction:Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.,PTM:Phosphorylated, probably by CDC2. The degree of phosphorylation varies throughout the cell cycle, and is highest at the G2\/M transition. Becomes hyperphosphorylated in response to cell differentiation and interaction with HES1 or RUNX1.,similarity:Belongs to the WD repeat Groucho\/TLE family.,similarity:Contains 6 WD repeats.,subcellular location:Nuclear and chromatin-associated, depending on isoforms and phosphorylation status. Hyperphosphorylation decreases the affinity for nuclear components.,subunit:Homooligomer and heterooligomer with other family members. Binds LEF1, RUNX1, RUNX3, FOXA2, KDM6A, UTY, histone H3, HESX1, ESRRG and the NF-kappa-B subunit RELA. Interacts with HES1 (via WRPW motif).,tissue specificity:In all tissues examined, mostly in brain, liver and muscle.,\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003eTLE1\/2\/3\/4 Polyclonal Antibody detects endogenous levels of TLE1\/2\/3\/4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eWestern Blot analysis of A549 3T3 mouse-liver SH-SY5Y K562 Hela 293T cells using TLE1\/2\/3\/4 Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200 | Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56187419427156,"sku":"ABM109669","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56187419459924,"sku":"ABM99805","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES8464-c-1.jpg?v=1782055326","url":"https:\/\/www.abmium.com\/products\/elk-es8464-tle1-2-3-4-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}