{"product_id":"elk-es7536-splicing-factor-1-phospho-ser82-rabbit-pab","title":"Splicing factor 1 (phospho Ser82) rabbit pAb","description":"\u003cp\u003eSplicing factor 1 (phospho Ser82) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Splicing factor 1.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eThis gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016],\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003ePhospho-Splicing factor 1 (S82) Polyclonal Antibody detects endogenous levels of Splicing factor 1 protein only when phosphorylated at S82.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eWestern blot analysis of SH-SY5Y 293T using p-Splicing factor 1 (S82) antibody. Antibody was diluted at 1:500 | Immunofluorescence analysis of A549 cells, using SF1 (Phospho-Ser82) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human brain, using SF1 (Phospho-Ser82) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from HUVEC cells treated with anisomycin 25ug\/ml 30', using SF1 (Phospho-Ser82) Antibody. The lane on the right is blocked with the phospho peptide.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56187292778836,"sku":"ABM109371","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56187292811604,"sku":"ABM99507","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES7536-c-1.jpg?v=1782052201","url":"https:\/\/www.abmium.com\/products\/elk-es7536-splicing-factor-1-phospho-ser82-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}