{"product_id":"elk-es7436-53bp1-phospho-ser25-rabbit-pab","title":"53BP1 (phospho Ser25) rabbit pAb","description":"\u003cp\u003e53BP1 (phospho Ser25) rabbit pAb is an ELK Biotechnology antibody for research workflows involving 53BP1.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003efunction:May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.,PTM:Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.,PTM:Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.,similarity:Contains 2 BRCT domains.,subcellular location:Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.,subunit:Interacts with IFI202A (By similarity). Binds to the central domain of TP53\/p53. May form homo-oligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with histone H4 that has been dimethylated at 'Lys-20'. Has low affinity for histone H4 containing monomethylated 'Lys-20'. Does not bind histone H4 containing unmethylated or trimethylated 'Lys-20'. Has low affinity for histone H3 that has been dimethylated on 'Lys-79'. Has very low affinity for histone H3 that has been monomethylated on 'Lys-79' (in vitro). Does not bind unmethylated histone H3.,\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003ePhospho-53BP1 (S25) Polyclonal Antibody detects endogenous levels of 53BP1 protein only when phosphorylated at S25.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eEnzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using 53BP1 (Phospho-Ser25) Antibody | Immunofluorescence analysis of NIH\/3T3 cells, using 53BP1 (Phospho-Ser25) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using 53BP1 (Phospho-Ser25) Antibody. The picture on the right is blocked with the phospho peptide.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56187285963092,"sku":"ABM109337","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56187285995860,"sku":"ABM99473","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES7436-c-1.jpg?v=1782051851","url":"https:\/\/www.abmium.com\/products\/elk-es7436-53bp1-phospho-ser25-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}