{"product_id":"elk-es7278-raf-b-phospho-thr753-rabbit-pab","title":"Raf-B (phospho Thr753) rabbit pAb","description":"\u003cp\u003eRaf-B (phospho Thr753) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Raf-B.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eThis gene encodes a protein belonging to the raf\/mil family of serine\/threonine protein kinases. This protein plays a role in regulating the MAP kinase\/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008],\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003ePhospho-Raf-B (T753) Polyclonal Antibody detects endogenous levels of Raf-B protein only when phosphorylated at T753.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eEnzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using B-Raf (Phospho-Thr753) Antibody | Western blot analysis of lysates from K562 cells treated with EGF 200ng\/ml 30', using B-Raf (Phospho-Thr753) Antibody. The lane on the right is blocked with the phospho peptide. | Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56187280392532,"sku":"ABM109287","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56187280425300,"sku":"ABM99423","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES7278-c-1.jpg?v=1782051623","url":"https:\/\/www.abmium.com\/products\/elk-es7278-raf-b-phospho-thr753-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}