{"product_id":"elk-es7161-cop1-phospho-ser387-rabbit-pab","title":"COP1 (phospho Ser387) rabbit pAb","description":"\u003cp\u003eCOP1 (phospho Ser387) rabbit pAb is an ELK Biotechnology antibody for research workflows involving COP1.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003edomain:The RING finger domain, in addition to its role in ubiquitination, functions as a structural scaffold to bring two clusters of positive-charged residues within spatial proximity to mimic a bipartite nuclear localization signal (NLS).,function:E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.,induction:By p53\/TP53.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the COP1 family.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 7 WD repeats.,subcellular location:In the nucleus, it forms nuclear speckles.,subunit:Homodimer. Homodimerization is mediated by the coiled coil domain.\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003ePhospho-COP1 (S387) Polyclonal Antibody detects endogenous levels of COP1 protein only when phosphorylated at S387.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eWestern blot analysis of lysates from K562 cells treated with UV 15', using RFWD2 (Phospho-Ser387) Antibody. The lane on the right is blocked with the phospho peptide.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56187275215188,"sku":"ABM109240","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56187275247956,"sku":"ABM99376","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES7161-c-1.jpg?v=1782051545","url":"https:\/\/www.abmium.com\/products\/elk-es7161-cop1-phospho-ser387-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}