{"product_id":"elk-es6982-raf-1-phospho-ser621-rabbit-pab","title":"Raf-1 (phospho Ser621) rabbit pAb","description":"\u003cp\u003eRaf-1 (phospho Ser621) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Raf-1.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eThis gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine\/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008],\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003ePhospho-Raf-1 (S621) Polyclonal Antibody detects endogenous levels of Raf-1 protein only when phosphorylated at S621.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eEnzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using C-RAF (Phospho-Ser621) Antibody | Immunofluorescence analysis of HeLa cells, using C-RAF (Phospho-Ser621) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using C-RAF (Phospho-Ser621) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from HeLa cells treated with UV 5', using C-RAF (Phospho-Ser621) Antibody. The lane on the right is blocked with the phospho peptide.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56187268825428,"sku":"ABM109177","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56187268858196,"sku":"ABM99313","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES6982-c-1.jpg?v=1782051383","url":"https:\/\/www.abmium.com\/products\/elk-es6982-raf-1-phospho-ser621-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}