{"product_id":"elk-es6605-plc-gamma1-phospho-tyr1253-rabbit-pab","title":"PLC γ1 (phospho Tyr1253) rabbit pAb","description":"\u003cp\u003ePLC γ1 (phospho Tyr1253) rabbit pAb is an ELK Biotechnology antibody for research workflows involving PLCG1.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eThe protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003ePhospho-PLC γ1 (Y1253) Polyclonal Antibody detects endogenous levels of PLC γ1 protein only when phosphorylated at Y1253.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eEnzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PLCG1 (Phospho-Tyr1253) Antibody | Western blot analysis of lysates from LOVO cells treated with and 293 cells treated with heat shock, using PLCG1 (Phospho-Tyr1253) Antibody. The lane on the right is blocked with the phospho peptide. | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56187240055124,"sku":"ABM109044","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56187240087892,"sku":"ABM99180","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES6605-c-1.jpg?v=1782051048","url":"https:\/\/www.abmium.com\/products\/elk-es6605-plc-gamma1-phospho-tyr1253-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}