{"product_id":"elk-es5717-histone-h3-3-phospho-ser31-rabbit-pab","title":"Histone H3.3 (phospho Ser31) rabbit pAb","description":"\u003cp\u003eHistone H3.3 (phospho Ser31) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Histone H3.3.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq, Jul 2008],\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003ePhospho-Histone H3.3 (S31) Polyclonal Antibody detects endogenous levels of Histone H3.3 protein only when phosphorylated at S31.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eEnzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Histone H3.3 (Phospho-Ser31) Antibody | Immunofluorescence analysis of HeLa cells, using Histone H3.3 (Phospho-Ser31) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56187193786708,"sku":"ABM108698","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56187193819476,"sku":"ABM98834","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES5717-c-1.jpg?v=1782050227","url":"https:\/\/www.abmium.com\/products\/elk-es5717-histone-h3-3-phospho-ser31-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}