{"product_id":"elk-es5669-grf-1-phospho-tyr1105-rabbit-pab","title":"GRF-1 (phospho Tyr1105) rabbit pAb","description":"\u003cp\u003eGRF-1 (phospho Tyr1105) rabbit pAb is an ELK Biotechnology antibody for research workflows involving GRF-1.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eThe human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008],\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003ePhospho-GRF-1 (Y1105) Polyclonal Antibody detects endogenous levels of GRF-1 protein only when phosphorylated at Y1105.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eWestern Blot analysis of 3T3 cells using Phospho-GRF-1 (Y1105) Polyclonal Antibody | Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GRF-1 (Phospho-Tyr1105) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from 293 cells treated with EGF 200ng\/ml 30', using GRF-1 (Phospho-Tyr1105) Antibody. The lane on the right is blocked with the phospho peptide.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56187191394644,"sku":"ABM108683","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56187191427412,"sku":"ABM98819","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES5669-c-1.jpg?v=1782050360","url":"https:\/\/www.abmium.com\/products\/elk-es5669-grf-1-phospho-tyr1105-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}