{"product_id":"elk-es5197-pyk2-phospho-tyr580-rabbit-pab","title":"PYK2 (phospho Tyr580) rabbit pAb","description":"\u003cp\u003ePYK2 (phospho Tyr580) rabbit pAb is an ELK Biotechnology antibody for research workflows involving PYK2.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eThis gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity t\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003ePhospho-PYK2 (Y580) Polyclonal Antibody detects endogenous levels of PYK2 protein only when phosphorylated at Y580.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eWestern Blot analysis of K562 cells using Phospho-PYK2 (Y580) Polyclonal Antibody | Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PYK2 (Phospho-Tyr580) Antibody | Immunohistochemistry analysis of paraffin-embedded human brain, using PYK2 (Phospho-Tyr580) Antibody. The picture on the right is blocked with the phospho peptide.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56187028406612,"sku":"ABM108477","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56187028439380,"sku":"ABM98613","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES5197-c-1.jpg?v=1782049754","url":"https:\/\/www.abmium.com\/products\/elk-es5197-pyk2-phospho-tyr580-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}