{"product_id":"elk-es5160-akt1-phospho-thr72-rabbit-pab","title":"Akt1 (phospho Thr72) rabbit pAb","description":"\u003cp\u003eAkt1 (phospho Thr72) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Akt1.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine\/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003ePhospho-Akt1 (T72) Polyclonal Antibody detects endogenous levels of Akt1 protein only when phosphorylated at T72.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eWestern Blot analysis of 3T3 cells using Phospho-Akt1 (T72) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Akt (Phospho-Thr72) Antibody | Immunohistochemistry analysis of paraffin-embedded human ovary, using Akt (Phospho-Thr72) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from NIH\/3T3 cells treated with TNF-a 20ng\/ml 30', using Akt (Phospho-Thr72) Antibody. The lane on the right is blocked with the phospho peptide.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56187026309460,"sku":"ABM108464","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56187026342228,"sku":"ABM98600","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES5160-c-1.jpg?v=1782049667","url":"https:\/\/www.abmium.com\/products\/elk-es5160-akt1-phospho-thr72-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}