{"product_id":"elk-es4404-hdac6-phospho-ser22-rabbit-pab","title":"HDAC6 (phospho Ser22) rabbit pAb","description":"\u003cp\u003eHDAC6 (phospho Ser22) rabbit pAb is an ELK Biotechnology antibody for research workflows involving HDAC6.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eHistones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation\/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase\/acuc\/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq, Jul 2008],\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003ePhospho-HDAC6 (S22) Polyclonal Antibody detects endogenous levels of HDAC6 protein only when phosphorylated at S22.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eImmunofluorescence analysis of Hela cell. 1,HDAC6 (phospho Ser22) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Galectin-3 Monoclonal Antibody(6G2)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min). | Western Blot analysis of 3T3 cells using Phospho-HDAC6 (S22) Polyclonal Antibody diluted at 1:500 | Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HDAC6 (Phospho-Ser22) Antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56186700169556,"sku":"ABM108122","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56186700202324,"sku":"ABM98258","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES4404-c-1.jpg?v=1782047285","url":"https:\/\/www.abmium.com\/products\/elk-es4404-hdac6-phospho-ser22-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}