{"product_id":"elk-es3719-yap-rabbit-pab","title":"YAP rabbit pAb","description":"\u003cp\u003eYAP rabbit pAb is an ELK Biotechnology antibody for research workflows involving YAP.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eThis gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013],\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003eYAP Polyclonal Antibody detects endogenous levels of YAP protein.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eWestern blot analysis of lysates from 1)Hela cell , 2)Hela cells knockdown by siRNA (F:GGUCAGAGAUACUUCUUAATT,R:UUAAGAAGUAUCUCUGACCTT), (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(Immunoway:RS23920)was diluted at 1:10000, 37° 1hour. (Red) GAPDH Monoclonal Antibody(5B7) (Immunoway:YM3029) antibody was diluted at 1:5000 as loading control, 4° over night, Dylight 680 secondary antibody(Immunoway:RS23710)was diluted at 1:10000, 37° 1hour. | Immunofluorescence analysis of Hela cell. 1,YAP Polyclonal Antibody(red) was diluted at 1:200(4° overnight). GAPDH Monoclonal Antibody(2B8)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min). | Immunofluorescence analysis of rat-spleen tissue. 1,YAP Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B | Immunofluorescence analysis of rat-spleen tissue. 1,YAP Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56186605797716,"sku":"ABM107627","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56186605830484,"sku":"ABM97763","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES3719-c-1.jpg?v=1782046098","url":"https:\/\/www.abmium.com\/products\/elk-es3719-yap-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}