{"product_id":"elk-es3119-p53-rabbit-pab","title":"p53 Rabbit pAb","description":"\u003cp\u003ep53 Rabbit pAb is an ELK Biotechnology antibody for research workflows involving p53.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003etumor protein p53(TP53) Homo sapiens This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013],\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003ep53 Polyclonal Antibody detects endogenous levels of p53 protein.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eWestern blot analysis of lysates from 1)p53 knockout A431 cell , 2)A431 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(Immunoway:RS23920)was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody(5B7) (Immunoway:YM3028) antibody was diluted at 1:5000 as loading control, 4° over night, Dylight 680 secondary antibody(Immunoway:RS23710)was diluted at 1:10000, 37° 1hour. | Immunofluorescence analysis of human-liver tissue. 1,p53 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B | Immunofluorescence analysis of human-liver tissue. 1,p53 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B | Immunofluorescence analysis of human-lung tissue. 1,p53 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56186528661844,"sku":"ABM107402","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56186528694612,"sku":"ABM97538","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES3119-c-1.jpg?v=1782045534","url":"https:\/\/www.abmium.com\/products\/elk-es3119-p53-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}