{"product_id":"elk-es2022-col2a1-rabbit-pab","title":"COL2A1 rabbit pAb","description":"\u003cp\u003eCOL2A1 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Collagen II.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eThis gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008],\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003eCOL2A1 Polyclonal Antibody detects endogenous levels of COL2A1 protein.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eWestern blot analysis of lysates from 1) k562 , 2) COLO205,3) MCF-7 cells, （Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. （Red） Actin β Monoclonal Antibody(5B7) (cat:YM3028) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:RS23710)was diluted at 1:10000, 37° 1hour. | Immunofluorescence analysis of rat-lung tissue. 1,COL2A1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B | Immunofluorescence analysis of rat-lung tissue. 1,COL2A1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B | Immunofluorescence analysis of rat-spleen tissue. 1,COL2A1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56186139902292,"sku":"ABM106827","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56186139935060,"sku":"ABM96963","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES2022-c-1.jpg?v=1782042942","url":"https:\/\/www.abmium.com\/products\/elk-es2022-col2a1-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}