{"product_id":"elk-es1593-actinin-alpha1-2-3-4-rabbit-pab","title":"Actinin-α1\/2\/3\/4 rabbit pAb","description":"\u003cp\u003eActinin-α1\/2\/3\/4 rabbit pAb is an ELK Biotechnology antibody for research workflows involving Actinin-α1\/2\/3\/4.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eAlpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003eActinin-α1\/2\/3\/4 Polyclonal Antibody detects endogenous levels of Actinin-α1\/2\/3\/4 protein.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eWestern Blot analysis of various cells using Actinin-α1\/2\/3\/4 Polyclonal Antibody diluted at 1:1000 | Western Blot analysis of A549 cells using Actinin-α1\/2\/3\/4 Polyclonal Antibody diluted at 1:1000 | Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Western blot analysis of lysates from 293, HeLa, and A549 cells, using ACTN1\/2\/3\/4 Antibody. The lane on the right is blocked with the synthesized peptide.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56185647333716,"sku":"ABM105612","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56185647366484,"sku":"ABM95748","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES1593-c-1.jpg?v=1782036783","url":"https:\/\/www.abmium.com\/products\/elk-es1593-actinin-alpha1-2-3-4-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}