{"product_id":"elk-es1444-msk1-phospho-ser376-rabbit-pab","title":"MSK1 (phospho Ser376) rabbit pAb","description":"\u003cp\u003eMSK1 (phospho Ser376) rabbit pAb is an ELK Biotechnology antibody for research workflows involving MSK1.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003ecatalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Appears to be activated by multiple phosphorylations on threonine and serine residues. ERK1\/2 and MAPK14\/p38-alpha may play a role in this process.,function:Serine\/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos\/FOS and c-jun\/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14).,miscellaneous:Enzyme activity requires the presence of both kinase domains.,PTM:Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain.,similarity:Belongs to the protein kinase superfamily. AGC Ser\/Thr protein kinase family.\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003ePhospho-MSK1 (S376) Polyclonal Antibody detects endogenous levels of MSK1 protein only when phosphorylated at S376.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eWestern Blot analysis of various cells using Phospho-MSK1 (S376) Polyclonal Antibody diluted at 1:1000 | Western blot analysis of lysates from HUVEC cells treated with PMA 125ng\/ml 30', using MSK1 (Phospho-Ser376) Antibody. The lane on the right is blocked with the phospho peptide. | Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56185465012564,"sku":"ABM104977","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56185465045332,"sku":"ABM95113","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES1444-c-1.jpg?v=1782035220","url":"https:\/\/www.abmium.com\/products\/elk-es1444-msk1-phospho-ser376-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}