{"product_id":"elk-es1321-gsk3alpha-beta-phospho-tyr279-216-rabbit-pab","title":"GSK3α\/β (phospho Tyr279\/216) rabbit pAb","description":"\u003cp\u003eGSK3α\/β (phospho Tyr279\/216) rabbit pAb is an ELK Biotechnology antibody for research workflows involving GSK3α\/β.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eglycogen synthase kinase 3 alpha(GSK3A) Homo sapiens This gene encodes a multifunctional Ser\/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011],\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003ePhospho-GSK3α\/β (Y279\/216) Polyclonal Antibody detects endogenous levels of GSK3α\/β protein only when phosphorylated at Y279\/216.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eImmunofluorescence analysis of Hela cell. 1,GSK3α\/β (phospho Tyr279\/216) Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min. | Immunofluorescence analysis of mouse-liver tissue. 1,GSK3α\/β (phospho Tyr279\/216) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B | Immunofluorescence analysis of mouse-liver tissue. 1,GSK3α\/β (phospho Tyr279\/216) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B | Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56185303236948,"sku":"ABM104433","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56185303269716,"sku":"ABM94569","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES1321-c-1.jpg?v=1782034204","url":"https:\/\/www.abmium.com\/products\/elk-es1321-gsk3alpha-beta-phospho-tyr279-216-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}