{"product_id":"elk-es1287-chk2-phospho-ser516-rabbit-pab","title":"Chk2 (phospho Ser516) rabbit pAb","description":"\u003cp\u003eChk2 (phospho Ser516) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Chk2.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eIn response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutati\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003ePhospho-Chk2 (S516) Polyclonal Antibody detects endogenous levels of Chk2 protein only when phosphorylated at S516.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eWestern Blot analysis of various cells using Phospho-Chk2 (S516) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). | Western blot analysis of lysates from HeLa cells treated with UV, using Chk2 (Phospho-Ser516) Antibody. The lane on the right is blocked with the phospho peptide.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56185254216020,"sku":"ABM104230","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56185254248788,"sku":"ABM94366","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES1287-c-1.jpg?v=1782033300","url":"https:\/\/www.abmium.com\/products\/elk-es1287-chk2-phospho-ser516-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}