{"product_id":"elk-es1076-his-tag-rabbit-pab","title":"His-tag rabbit pAb","description":"\u003cp\u003eHis-tag rabbit pAb is an ELK Biotechnology antibody for research workflows involving His-tag.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eThe his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003eHis-tag Polyclonal Antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eWestern Blot analysis using His-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing His tag (1) and untransfected (2). Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western Blot analysis of HIS-protein cells using His-tag Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56184954061140,"sku":"ABM102902","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56184954093908,"sku":"ABM93038","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ES1076-c-1.jpg?v=1782028224","url":"https:\/\/www.abmium.com\/products\/elk-es1076-his-tag-rabbit-pab","provider":"ABMIUM","version":"1.0","type":"link"}