{"product_id":"elk-em1000-flag-tag-mouse-mab","title":"Flag-Tag Mouse mAb","description":"\u003cp\u003eFlag-Tag Mouse mAb is an ELK Biotechnology antibody for research workflows involving Flag-Tag.\u003c\/p\u003e\u003ch3\u003eBackground\u003c\/h3\u003e\u003cp\u003eThe DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.\u003c\/p\u003e\u003ch3\u003eAdditional antibody information\u003c\/h3\u003e\u003ctable\u003e\u003ctbody\u003e\u003ctr\u003e\n\u003cth\u003eValidation evidence captions\u003c\/th\u003e\n\u003ctd\u003eIP antibody use：5ug Flag Mouse IgG1 per ml Lysate，WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with Flag-tag fusion protein 3、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 4、IP (transfected 293+anti- Flag mAb+ P | 1ug Flag fusion protein+ Primary antibody dilution at 1:10,000. | IF analysis of 293 cells transfected with a Flag-tag protein, using ELKbio anti-FlagTag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-Flag)\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\u003c\/table\u003e","brand":"ELK Biotechnology","offers":[{"title":"Unconjugated \/ 100µL","offer_id":56184854610260,"sku":"ABM102230","price":220.41,"currency_code":"GBP","in_stock":true},{"title":"Unconjugated \/ 50µL","offer_id":56184854643028,"sku":"ABM92366","price":131.54,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/EM1000-c-1.jpg?v=1782026642","url":"https:\/\/www.abmium.com\/products\/elk-em1000-flag-tag-mouse-mab","provider":"ABMIUM","version":"1.0","type":"link"}