{"product_id":"elk-elk8156-12-hete-12-hydroxyeicosatetraenoic-acid-elisa-kit","title":"12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit","description":"\u003cp\u003e12-HETE(12-Hydroxyeicosatetraenoic Acid) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of 12-HETE.\u003c\/p\u003e\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 12-HETE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 12-HETE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 12-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include 12HETE.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56184202101076,"sku":"ABM78869","price":236.41,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56184202133844,"sku":"ABM78868","price":334.17,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56184202166612,"sku":"ABM78870","price":1420.22,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK8156-b-1.jpg?v=1782012148","url":"https:\/\/www.abmium.com\/products\/elk-elk8156-12-hete-12-hydroxyeicosatetraenoic-acid-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}