{"product_id":"elk-elk8091-dhvd3-1-25-dihydroxyvitamin-d3-elisa-kit","title":"DHVD3(1,25-Dihydroxyvitamin D3) ELISA Kit","description":"\u003cp\u003eDHVD3(1,25-Dihydroxyvitamin D3) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of DHVD3.\u003c\/p\u003e\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHVD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include DH-VD3; Calcitriol; Rocaltrol; Calcijex; 1,25-(OH)2D3; 1,25-Dihydroxycholecalciferol; 1,25-Dihydroxy Vitamin D3.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56184192565588,"sku":"ABM78695","price":236.41,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56184192598356,"sku":"ABM78694","price":334.17,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56184192631124,"sku":"ABM78696","price":1420.22,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK8091-b-1.jpg?v=1782012085","url":"https:\/\/www.abmium.com\/products\/elk-elk8091-dhvd3-1-25-dihydroxyvitamin-d3-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}