{"product_id":"elk-elk7953-cattle-il-2sra-cd25-soluble-interleukin-2-receptor-alpha-chain-elisa-kit","title":"Cattle IL-2sRa\/CD25(Soluble Interleukin-2 Receptor alpha chain) ELISA Kit","description":"\u003cp\u003eCattle IL-2sRa\/CD25(Soluble Interleukin-2 Receptor alpha chain) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of IL-2sRa\/CD25.\u003c\/p\u003e\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle IL-2sRa\/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle IL-2sRa\/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle IL-2sRa\/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle IL-2sRa\/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include IL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56184154980692,"sku":"ABM78284","price":366.17,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56184155013460,"sku":"ABM78283","price":522.59,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56184155046228,"sku":"ABM78285","price":2221.88,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK7953-b-1.jpg?v=1782011744","url":"https:\/\/www.abmium.com\/products\/elk-elk7953-cattle-il-2sra-cd25-soluble-interleukin-2-receptor-alpha-chain-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}