{"product_id":"elk-elk7890-hvd3-25-hydroxyvitamin-d3-elisa-kit","title":"HVD3(25-Hydroxyvitamin D3) ELISA Kit","description":"\u003cp\u003eHVD3(25-Hydroxyvitamin D3) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of HVD3.\u003c\/p\u003e\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with HVD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to HVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include 25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56183818191188,"sku":"ABM78113","price":236.41,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56183818223956,"sku":"ABM78112","price":334.17,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56183818256724,"sku":"ABM78114","price":1420.22,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK7890-b-1.jpg?v=1782005578","url":"https:\/\/www.abmium.com\/products\/elk-elk7890-hvd3-25-hydroxyvitamin-d3-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}