{"product_id":"elk-elk7866-3-nt-3-nitrotyrosine-elisa-kit","title":"3-NT(3-Nitrotyrosine) ELISA Kit","description":"\u003cp\u003e3-NT(3-Nitrotyrosine) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of 3-NT.\u003c\/p\u003e\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 3-NT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 3-NT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-NT in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include 3-Nitro-L-Tyrosine; 3-Nitrotyrosine; Nitrotyrosine.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56183811834196,"sku":"ABM78038","price":236.41,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56183811866964,"sku":"ABM78037","price":334.17,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56183811899732,"sku":"ABM78039","price":1420.22,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK7866-b-1.jpg?v=1782005554","url":"https:\/\/www.abmium.com\/products\/elk-elk7866-3-nt-3-nitrotyrosine-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}