{"product_id":"elk-elk7839-bhb-beta-hydroxybutyric-acid-elisa-kit","title":"bHB(Beta-Hydroxybutyric Acid) ELISA Kit","description":"\u003cp\u003ebHB(Beta-Hydroxybutyric Acid) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of bHB.\u003c\/p\u003e\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with bHB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to bHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of bHB in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include OHB; Beta-Hydroxybutyrate; 3-Hydroxybutyric Acid.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56183807017300,"sku":"ABM77966","price":236.41,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56183807050068,"sku":"ABM77965","price":334.17,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56183807082836,"sku":"ABM77967","price":1420.22,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK7839-b-1.jpg?v=1782004765","url":"https:\/\/www.abmium.com\/products\/elk-elk7839-bhb-beta-hydroxybutyric-acid-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}