{"product_id":"elk-elk6672-human-sema5a-semaphorin-5a-elisa-kit","title":"Human SEMA5A(Semaphorin 5A) ELISA Kit","description":"\u003cp\u003eHuman SEMA5A(Semaphorin 5A) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of SEMA5A.\u003c\/p\u003e\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SEMA5A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SEMA5A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SEMA5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SEMA5A in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include SEMAF; SemF; Semaphorin-F; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 5A.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56183361405268,"sku":"ABM74012","price":284.4,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56183361438036,"sku":"ABM74011","price":407.05,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56183361470804,"sku":"ABM74013","price":1731.29,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK6672-b-1.jpg?v=1782001831","url":"https:\/\/www.abmium.com\/products\/elk-elk6672-human-sema5a-semaphorin-5a-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}