{"product_id":"elk-elk5700-dog-et-1-endothelin1-elisa-kit","title":"Dog ET-1(Endothelin1) ELISA Kit","description":"\u003cp\u003eDog ET-1(Endothelin1) ELISA Kit is an ELK Biotechnology ELISA kit for research measurement of ET-1.\u003c\/p\u003e\u003ch3\u003eTest principle\u003c\/h3\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/p\u003e\u003ch3\u003eTarget background\u003c\/h3\u003e\u003cp\u003eAlternative names include ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1.\u003c\/p\u003e","brand":"ELK Biotechnology","offers":[{"title":"48T","offer_id":56182571401556,"sku":"ABM70823","price":366.17,"currency_code":"GBP","in_stock":true},{"title":"96T","offer_id":56182571434324,"sku":"ABM70822","price":522.59,"currency_code":"GBP","in_stock":true},{"title":"96T*5","offer_id":56182571467092,"sku":"ABM70824","price":2221.88,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1040\/1782\/2036\/files\/ELK5700-b-1.jpg?v=1781997772","url":"https:\/\/www.abmium.com\/products\/elk-elk5700-dog-et-1-endothelin1-elisa-kit","provider":"ABMIUM","version":"1.0","type":"link"}